| IKZF3

Zinc finger protein Aiolos (IKZF3), also known as Ikaros family zinc finger protein 3, is a protein encoded by the IKZF3 gene. This gene encodes a member of the Ikaros zinc finger protein family. The three members of this protein family (Ikaros, Aiolos, and Helios) are hematopoiesis-specific transcription factors involved in regulating lymphocyte development. The gene product is a transcription factor that plays a crucial role in regulating the proliferation and differentiation of B lymphocytes. It has been demonstrated to interact with BCL2-like 1 and HRAS.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	1600	800	400	200	100	50	25	0
OD	2.22	1.56	0.92	0.63	0.42	0.25	0.18	0.08
Corrected OD	2.14	1.48	0.94	0.55	0.34	0.17	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human IKZF3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	95
Cell Lysates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human IKZF3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	90-110
	Average (%)	91	96
1 : 4	Range (%)	89-103	104-114
	Average (%)	94	107